FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10331392
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGATC489642447.393652278415146Illumina Single End PCR Primer 1 (97% over 34bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGAT1160431.1232077923284685Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGATCT637650.6171965984835345Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGAT326250.3157851333102064Illumina Single End PCR Primer 1 (97% over 34bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGA250220.2421938882969497Illumina Single End PCR Primer 1 (97% over 34bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGTAGATC222300.21516945635205786Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)