FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6962457
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAACTGGGTGTAGATC1861152.673122433646628Illumina Single End Sequencing Primer (100% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAACTGGGTGTAGATCT157660.2264430502048343Illumina Single End Sequencing Primer (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)