FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120504
Total Bases768 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGATGTCCGTGTAGATC1772433.461436608583843Illumina Single End PCR Primer 1 (97% over 37bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGATGTCCGTGTAGATCT63620.12424558207551444Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)