FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6369979
Total Bases955.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGATC281637044.21317558503725Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGATCT668261.0490772418558993Illumina Single End PCR Primer 1 (97% over 34bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGAT139270.21863494369447686Illumina Single End PCR Primer 1 (97% over 35bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGATC125400.19686093156665038Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAGAGCGTCGTGTAGGGAAAGAGTGTTGGAGTTGGTGTAGATCT77330.12139757446610108Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)