FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008408134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008408134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65157825
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3691720.566581220290886No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC3648790.559992602576897No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC2167980.3327274966590736No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1489580.22861106858615984No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1210130.1857228966743442No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1145760.17584380694106963No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1049890.16113030169438589No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1009780.15497447927397823No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA970260.14890920622350423No Hit
CCCCAGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA924100.14182486907750527No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG725600.11136037766760938No Hit
CACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTT712270.1093145758011413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)