FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008408994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008408994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51248849
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2011550.39250637609441724No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1518220.296244702002966No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA1009960.1970697917527865No Hit
CTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTC982090.19163162083893825No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCT872790.170304312590513No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACG863060.1684057333658362No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG842420.16437832584298626No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCA725540.1415719599868477No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAA663180.1294038818315705No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC605140.1180787494368898No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT517920.10105983063151332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)