FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008409584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008409584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59875831
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length76-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3296520.5505593734473598No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA2681280.44780672856131215No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1356760.22659560248942515No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1184980.1979062303118599No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA1122060.18739781665827734No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG936880.15647047971659886No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA823290.1374995530333433No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA744570.12435234510565707No Hit
CTGGCCTACTGGGCTGACATTAACTACAATTATGGGAAATGCAAAAGTTG669500.11181473205774799No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA630560.1053112732581532No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTC606990.10137479344545547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)