FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008410578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008410578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59962277
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA2514260.4193069586066587No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA1883950.3141892026548625No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1603760.26746149083030984No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG1592660.2656103269727399No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1276180.2128304767345643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1196010.1994604040803854No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC979360.1633293545540307No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGGGGAGTGAGCACACACTCGG953980.15909669340942473No Hit
GAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA932630.15553612148517976No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT885800.1477262112644588No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC868410.14482605455426584No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG847220.14129216607301287No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG787320.13130255210288294No Hit
CAGAAGTGGATTCAGTGAATCTAGGAAGACAGCAGCAGACAGGATTCCAG767750.12803883348192396No Hit
CAGTAACACTTCCGGTGACTTTGAGAAAGATGATGATGCCTGTAAGACTG765830.12771863216601997No Hit
GCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAG764640.12752017405876698No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT748930.12490019349999No Hit
CATTAATTGACAGCTGACCCAGGTGCTACACAGAAGTGGATTCAGTGAAT738620.12318077914219301No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG735770.12270548031389802No Hit
ATTTAATTTCTGGTGGTGCAGAAGTTAGAAGGTAAAGCTTGAGAAGATGA734310.12246199389659602No Hit
CTCAGATCTTTGTCCCAGGCCAGGAATCTTTTAATGACAGGATCCTCTGT695390.11597124638879207No Hit
CCGAACCCAAGAGGCGGAGGTTGAGCTGAGAAGAGGCCAGTGCACTCAAG693330.11562769706027008No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG690770.11520076197239808No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA690140.11509569591561708No Hit
GCCAGGAAGAAATGAATGCGTTGGTATTGCCAGGAGGGGAGGCCGGCCCG686190.11443694841675209No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT658890.10988408595624212No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT657570.10966394755155813No Hit
AGTGAATCTAGGAAGACAGCAGCAGACAGGATTCCAGGAACCAGTGTTTG638370.10646193439251815No Hit
TTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGT628780.10486259552818515No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT628700.10484925380668916No Hit
CACAGAAGTGGATTCAGTGAATCTAGGAAGACAGCAGCAGACAGGATTCC628170.10476086490177816No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA626870.10454406192746815No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGGGGAGTGAGCACACACTC619900.10338166444212918No Hit
TCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT615400.10263119260797919No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA600710.1001813189982762No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)