FastQCFastQC Report
Wed 29 May 2024
EGAF00008431266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9890087
Total Bases741.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCGGTGCCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA129700.13114141463062964RNA PCR Primer, Index 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)