FastQCFastQC Report
Wed 29 May 2024
EGAF00008431274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10604914
Total Bases795.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT182600.1721843288875327RNA PCR Primer, Index 11 (100% over 41bp)
CCCCAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT154480.1456683194224866RNA PCR Primer, Index 11 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)