FastQCFastQC Report
Wed 29 May 2024
EGAF00008431304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9568644
Total Bases717.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGCTGCCACCATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC409870.42834700507198303RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC185210.19355929638515135RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC173110.18091382645231655RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC144360.15086777185983719RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC116760.12202355945105701RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC114290.11944221145650313RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC110690.11567992288144485RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC98720.10317031336937606RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC98190.1026164208847147RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC96080.10041130174766665RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)