FastQCFastQC Report
Wed 29 May 2024
EGAF00008431317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948091
Total Bases596.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGCTGCCACCATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC228790.2878552849986242RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109280.13749213490384044RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC105090.13222042877969062RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC95970.12074597535433351RNA PCR Primer, Index 1 (100% over 31bp)
CCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT94170.1184812805993288RNA PCR Primer, Index 32 (100% over 41bp)
CCACCGCTGCCACCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC90170.11344862558820727RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)