FastQCFastQC Report
Wed 29 May 2024
EGAF00008431324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10351183
Total Bases776.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCGGTGCCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT149010.14395456055602535RNA PCR Primer, Index 3 (100% over 34bp)
CCGGACGAGCCCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT120170.11609301081818377RNA PCR Primer, Index 3 (100% over 34bp)
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC117850.11385172110279569RNA PCR Primer, Index 1 (100% over 31bp)
CCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATAT103800.10027839330055319RNA PCR Primer, Index 44 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)