FastQCFastQC Report
Wed 29 May 2024
EGAF00008431325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7603488
Total Bases570.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGCTGCCACCATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC246900.32471939194222443RNA PCR Primer, Index 1 (100% over 31bp)
CCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAT108320.1424609337188406RNA PCR Primer, Index 17 (100% over 41bp)
CCACCGCTGCCACCACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC77720.10221624601761718RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)