FastQCFastQC Report
Wed 29 May 2024
EGAF00008431343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8238398
Total Bases617.8 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTTCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC117100.14213928484639854RNA PCR Primer, Index 1 (100% over 31bp)
CCACTTCTGACACCATTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC103580.12572832727916278RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCATTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC88220.10708392578265823RNA PCR Primer, Index 1 (100% over 31bp)
CCAGCGGTGCCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT82830.10054139166376765RNA PCR Primer, Index 3 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)