FastQCFastQC Report
Wed 29 May 2024
EGAF00008431366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13384678
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC307060.22941157045391755RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC164390.12281954037295481RNA PCR Primer, Index 1 (100% over 31bp)
CGGGTGCCCCCTCCTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA139150.10396215732645941RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)