FastQCFastQC Report
Wed 29 May 2024
EGAF00008431394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95006534
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATCACACCGTTA6782650.7139140556374786No Hit
CCTCCCTTGGCTCAGC6662080.7012233495435166No Hit
ACGGCTTCTATTCGGT6486840.6827783023849707No Hit
GTAATCTCTCATCTTT6072590.6391760381449132No Hit
CCCTGATTGTCCCAGG6020620.6337058880602885No Hit
AGAACATGATACGTTG5958810.6272000197375899No Hit
GTTATCACTATAGGTA5950110.6262842932466097No Hit
TCCGTTGACCTCACTC5944770.6257222266418013No Hit
TAGTACCGATCGTCAA5904200.621451994028116No Hit
AGGGAGTTGTACCTGA5762740.6065624917966169No Hit
ACTACCCACCGACTAT5758030.6060667364204656No Hit
AAGTACCACAGCTGAG5752620.6054973019013619No Hit
ATCCAGCCTTCTGGAT5651300.5948327722385914No Hit
CGCTTGTACCGAAGCA5556490.5848534586052787No Hit
CACCGTTACGGGTTCC5550270.5841987667921872No Hit
CGATCGGTGACCAAGG5335170.5615582187220933No Hit
CGTGCCAGAAATCCAC5326350.5606298615208929No Hit
TGTGCAATGTCGTCAA5323830.5603646166062641No Hit
GTTGATGACGAAGGTC5309220.5588268276369286No Hit
CCATCCATGCATACTG5234770.5509905245043462No Hit
CGATATTCTAACGAAT5147290.5417827367536636No Hit
AAAGATCGACTTTGTT5115750.5384629650840647No Hit
TTGGTCCACTGCTTGA5039940.5304835139023175No Hit
AGCTGATACAGCTTTG4976070.5237608183875017No Hit
GATAGGTCTCCTGAGC4965990.5226998387289867No Hit
ATCCAGCCTTACGATC4839270.5093618087362286No Hit
ACGTCCACTATCCTAG4682190.49282820905770547No Hit
TCCATTTGAGGCCTTG4624150.48671915554776474No Hit
TCTAGTGGACACCTAA4582780.4823647181992767No Hit
CACTTTATGGTCTTGA4568030.4808121934013507No Hit
GAAGGACACATCCTAG4500790.4737347854411782No Hit
GTTATTGCTTTAGAAG4372460.4602272934196294No Hit
ACCAAGTTGTAAGTAG4241200.44641140155686554No Hit
GGTAGTTTGCATAGCT4228230.4450462322938757No Hit
GAACAAATGAGGTGCA4195730.44162541494251334No Hit
ATCAAGCACGCTGTTA4189430.4409623026559416No Hit
CGGTCGATGGGACCAA4154200.4372541366470647No Hit
GGAGTTACTTTTCTTG4145750.43636472413571054No Hit
AGCTGCTCTAAGGCCA4135080.4352416434852786No Hit
TTGATTCACCGTTAGT4070450.4284389534724002No Hit
TTGCGATGAAGCAATA4068930.4282789644762749No Hit
TCCGCGAGACATTCAG4067850.4281652880842911No Hit
TAGGGACGATTGACCA4054010.4267085461722032No Hit
TACTAGCGATAGGCGA3968410.4176986395483073No Hit
AACGAGGTGGCACTTA3956850.4164818811304073No Hit
GTTACAGTGAAATGCA3955580.41634820611390794No Hit
CTGTGCACTGGACCTC3950930.41585876609286687No Hit
TACGTTTACGTCTGGC3940660.4147777878098363No Hit
TCGGACCGATGGGCCT3898380.41032756757551014No Hit
TGGTAGGGAACGCGAA3836690.40383432996303176No Hit
GAGCCTCGAAGTGGTG3832070.4033480476195458No Hit
GTCGACCGATGGCATT3822580.40234916895294803No Hit
CAGGCTTCTAGGACAC3813360.4013787093843461No Hit
CCACGGATGAGGACCA3759480.39570752049537977No Hit
CCACTCTGATAGGCGA3722480.3918130515107519No Hit
CACTTAGGAGATCATC3701550.3896100451364745No Hit
AGAACAATGTGCACCG3694980.3889185137519068No Hit
AAGACGGTGAGCAAGC3693390.3887511568414863No Hit
GGAGTTACTCGCACTG3671810.3864797341201817No Hit
GTAGTGGGACATTCAG3661710.3854166493432968No Hit
GGTACCTCTCTTCACC3650000.38418410253762125No Hit
GTGGTTAACCCGATTA3617900.3808053875536602No Hit
GTCCTGACTCGGTTTA3614870.3804864621205948No Hit
GGTGGAAACAACCCGC3613830.3803769959653512No Hit
CCGCAAGTGGAGAGGG3613730.3803664703735008No Hit
TATCCATACCCGTGAC3610370.3800128104873292No Hit
TGCGGGACTAATCCGT3605020.3794496913233357No Hit
TATGTACCTAGGTGAC3572230.3759983497556073No Hit
GCTAAGACTGAGAGGG3556670.3743605676636935No Hit
TCCATTTGATTCGTTT3555450.37423215544311933No Hit
GGGAAAGCTAAATCGA3530210.37157549606009205No Hit
CAGATACTGCGACATT3482630.3665674194576975No Hit
CTTGACGCTCCTTTGC3460050.36419074081788944No Hit
CCTAGCACTGCGCACT3445620.36267189791388454No Hit
GCCGAACTGTTCGGGC3420710.3600499729839634No Hit
GTGATAAGAATCAGGG3418620.3598299881142912No Hit
GGTATCTGAGAATCTC3392490.35707965096379585No Hit
AAAGCCTTGTGAACAA3377020.3554513419045473No Hit
TACAACCGACCAGAGA3332640.35078008424136387No Hit
TCCGACACTAGCCTAT3282380.3454899217773801No Hit
TGCTAGTGAACAACAA3195400.3363347619859493No Hit
CGTGTCTACCGTAACT3157330.33232766916852263No Hit
ATGGACTACGACGCCT3145430.3310751237383315No Hit
TGGGTGCACGGTTGAG3121440.3285500342534336No Hit
GGAATGGTGCTTAGTG3095940.3258660083315954No Hit
GAGAGAAGACGAGGAT3049910.3210210784028812No Hit
TCAACATCTCATGATC3026500.3185570373507153No Hit
CCAGGCAACTGTTCCC3004350.31622561875586364No Hit
TCGCTGGGAGCTAGAG2992990.3150299115216644No Hit
ATGATGCACTCCGTAA2980250.31368895111993034No Hit
GTTCCCAACCAATGAA2920110.3073588601811324No Hit
TGTTAACCTTCTTTGT2911500.30645260672281766No Hit
ACAGCCGACACGTAAA2842210.299159424129713No Hit
TACACGTGATGCATCA2839920.2989183880763401No Hit
AAGGGCATGACGCGAA2829740.29784688282597493No Hit
CGTCGTACTAGCTTGT2813220.29610805505229776No Hit
TCCCAGACTGAGAGGG2792250.2939008384412803No Hit
TTCGCCAACATTGGTC2780730.29268829026012044No Hit
GCTTTAGGAAGCAGGT2776420.29223463725137055No Hit
CGCGATCGAGAGAGGG2748800.28932746878230503No Hit
TAACGAAGAGGATGGT2741010.2885075251771631No Hit
CAGGGCACTACCAGTT2735500.28792756506620903No Hit
ATTTCGACTTGGCTGC2725680.2868939519465051No Hit
AGGAAGAGAACACCGC2724870.28680869465251724No Hit
GGGTATGGATCGAGAG2723850.28670133361564376No Hit
GGGCTGACTGTCAATC2680750.2821648035281447No Hit
GCTAGTCCTGAGCTGA2678000.2818753497522602No Hit
CCTTGGTACCGACAAG2665730.28058385963222277No Hit
GAGATCACTGCGTATT2662700.2802649341991573No Hit
TCTTTCCACACGCCAC2649660.27889239702187224No Hit
CTGAGCGTGAGTGAGC2646470.27855663064184616No Hit
CTATCTATGCACTCAA2630660.2768925345703065No Hit
GTCACAATGGAAATCA2622450.2760283834793931No Hit
AATGATGGAAGGCATG2621300.27590733917311416No Hit
ATAGGCGGAGGTATGG2618050.2755652574379779No Hit
ACTCTAGGAAGTCTAC2599140.2735748680190775No Hit
TCGTATGGATCATTGG2590800.2726970336587587No Hit
TACCTCGACAAAGTAC2543200.2676868519379941No Hit
TCACGGGACAAGGTGC2509910.26418288241101395No Hit
TAGCATTCTGTGAGCA2506440.26381764437380695No Hit
GTATTCTACCTTAGTG2493410.2624461597557069No Hit
TACCTCTCTAAACATG2486680.26173778742417864No Hit
GGGGGGGGGGGGGGGG2482850.2613346572563104No Hit
TGTTAACCTCCAGCAC2480750.2611136198274531No Hit
CTAGCTCACTTGACAC2473930.26039577446325957No Hit
ATTACCTACAGATTCC2459320.258857985493924No Hit
CATCTGAGACGCCACA2454180.2583169700728163No Hit
TTACTGGGATTTCTTG2450410.25792015526005824No Hit
CATTGGAACCCAACGC2434260.25622027217622734No Hit
GGAAGGACTTGAGCAG2431700.2559508170248585No Hit
GAGAGGTTGCCTAACA2405650.25320890034784344No Hit
TTCCTACCTTTCTGGA2379010.2504048826789113No Hit
CTGACGTCTTTGTTTC2370570.24951652272674216No Hit
CTTTCTGCTTGGAACG2360930.24850185567236882No Hit
CATAGGTACTCGTAGT2358770.24827450288840133No Hit
TAATTGCCTTCCCGTT2357890.24818187768011832No Hit
GCTAGTCCTAAAGCTA2351220.24747982070370023No Hit
CAAACCATGAAAGTAC2344120.2467325036823257No Hit
CTTCCTACTCCTTCCA2320240.24421899234846312No Hit
AATGGGTCTCCTAACA2317870.2439695358216099No Hit
TACCGCTACACGCCAC2316610.24383691336429553No Hit
TGGCCTGGACGTACAT2289120.2409434281646355No Hit
TGTAAGCTGGAGCTGA2258700.23774154312376033No Hit
GTAACTGTGACACCTA2255090.2373615692579628No Hit
CAAGAGTCTGCCGCTA2254450.23729420547012062No Hit
TCACCTGACAGCAATA2250610.23689002274306734No Hit
TTACACACTCGAGGTA2234040.23514593217346505No Hit
GAGGTTGGAGAATGTA2211760.232800830309208No Hit
CAATGTGTGTACAGCT2197160.23126409389905753No Hit
CTGAGATACCCGGCTA2195300.23106831789064108No Hit
GGCACACACATCCTTT2187140.23020942959565285No Hit
GGGTATACTACCGTGC2186770.2301704849058066No Hit
AGCTTCCACTGAACAA2171520.22856533214862884No Hit
TTAGCCAGACAAGTTC2169050.2283053500299253No Hit
GATCCAAACATCCGTA2151110.22641705885197327No Hit
GGTCAGAGACTTTGTT2148100.22610023853727787No Hit
CAAGAAATGCATCCTC2147350.22602129659840026No Hit
TAAGCCAACCTCATCC2136040.2248308521601262No Hit
ACCTACCTGTTCAGTG2129520.22414458357148362No Hit
GAGCCTGACACGCGAA2108720.2219552604666117No Hit
TGTAAGCTGCCGCAGT2093420.22034484491350878No Hit
TTGTATCTGGTTAGGG2087700.21974278105966902No Hit
AACAATGGACCTTCCA2086410.21960700092479957No Hit
GCTGAATTGCGGCTCA2084720.2194291184225287No Hit
TGTTGACGAGGAATAT2072040.21809447337590485No Hit
ATTAGCGTGTAATAGG2024420.21308218653677022No Hit
GGACATTTGTCATTGG2021830.21280957370784626No Hit
TCACCCATGCGGTACC2015790.21217382796008535No Hit
GTCACCTACGCTAGAG2013220.21190332024953146No Hit
AGCGCCTGATTTCCGC2007640.21131599222428218No Hit
GTTGCTAGATGCGGCT2005210.21106022034231875No Hit
GGAAGGACTGAAGGCT2001850.21070656045614716No Hit
CGCGTTGGACGACAAG1993740.2098529349570841No Hit
GATGAATGAAGCCTAT1991990.20966873709970307No Hit
TGTAGACACAAACTGA1964560.2067815672551532No Hit
TGAGAATGACTCATCC1953740.20564269821694578No Hit
AACTCGATGTCAATGC1952810.20554481021273757No Hit
CTGCTAGCTAGACAAT1949990.20524798852255785No Hit
GGATACTACACCAAGG1946290.20485854162409506No Hit
ATACCTGGATCGTCAA1933670.20353021193258142No Hit
CAGCCGATGAGTACGG1914760.20153982251368102No Hit
TTGGTATCTGAACGTG1909790.20101670059871884No Hit
TGTTGTACTAGGGCTA1893380.1992894509760771No Hit
AAGGATCCTAGTCTAC1885170.1984252998851637No Hit
CTGCACGGAACAGATA1881230.19801059156626005No Hit
ATCTCTAACCAGATTC1873990.197248538716295No Hit
CACATGATGCGAGGAT1861610.1959454704452222No Hit
CCGTTACTGATCATCG1850290.19475397344776307No Hit
ATAGGCGGAACCGTTA1843410.1940298127284593No Hit
GCTATTGGACTTTGTT1838700.193534057352308No Hit
CTGGTGGTGCCAAACT1829080.19252149541630473No Hit
CCTCACAACGCACTTA1828260.1924351855631319No Hit
GGATGCCTGCCCGAGT1825620.19215730993828278No Hit
TGGGTGCACTTCGTTT1806510.19014586933568167No Hit
AGGTAGGTGGACTCGC1793020.18872596699507005No Hit
CTATGGATGCAACGCC1790030.1884112517987447No Hit
TCAGGCTCTGTGAGCA1779350.18728711858912778No Hit
CGAATTTCTAGCTTGT1778560.18720396641351006No Hit
CTGACGTCTCCTAGGC1762560.1855198717174547No Hit
CTCAATTTGTAATGCC1752380.1844483664670895No Hit
TTGGCGCGATCTGCAA1750470.1842473276627479No Hit
CCTACGGGAAAACTGA1746000.18377683370703746No Hit
TTCTAGTTGGTCTTGA1744850.18365578940075847No Hit
CATGGAGGATACAGAA1741830.18333791652687803No Hit
TGTCGCTTGCGATTCA1731930.1822958829336938No Hit
ACGTGTTGACTGAGAG1724220.18148435980203215No Hit
GGACGAATGGCTTACA1700190.17895506008039405No Hit
AGTCCCACTGCCACGT1690360.17792039440150506No Hit
GTGATAAGAACTAGGG1688560.17773093374819882No Hit
CCACATCACAAGTCTC1684840.17733938173136599No Hit
AGAGCAGTGTTGGTTG1683350.17718255041279582No Hit
CTCAAACGAAGTTGAA1668810.17565212935775554No Hit
CGATGATTGCCTGAGC1668730.17564370888427527No Hit
TAGTGAAGAAACCAAA1651000.17377752144920897No Hit
TGCAATGTGCGTTCTC1645490.17319756133825492No Hit
AGCTATCGAAAACTGA1643660.17300494300739355No Hit
ACAGTTGACTCAGGTT1638570.17246919038221098No Hit
TGGACATACACCAGTT1630310.17159977649537242No Hit
AAGTGGCGACCAGAGA1618550.17036196689377175No Hit
CGAACATACGCCCTGA1618260.17033144267740574No Hit
CAGAGTACTAAAGTAC1616840.17018197927313083No Hit
CCCGAGTGATGTGCTA1616600.17015671785269001No Hit
TGAGAATGAAATGCGG1616120.17010619501180835No Hit
TCGACACCTGCTAGAG1608830.16933887936591815No Hit
GAAGCAGTGAAGGCTG1604230.16885470214080223No Hit
AGATATGGATACTTTC1577970.1660906817209014No Hit
TACCGCTTGGAACGTG1574640.1657401795122849No Hit
CGTCTTGACTCTGTGA1551150.1632677179866387No Hit
ACGGCCTGAACCAGTT1536680.16174466484589364No Hit
GATAAGAACTAGACAA1535070.16157520281710308No Hit
CGACGTTCTTGGCAAG1527710.16080051925691763No Hit
TCGACTCTGTTGCGTA1519380.15992373745578384No Hit
TTCCTAAACCACCTAA1518260.15980585082705995No Hit
AAACAACGATAACGAA1517710.15974796007188305No Hit
TCGGACCGATAGGTTC1509960.15893222670348126No Hit
AGTCGCGTGTCGTACA1505670.15848067881310143No Hit
CCATCCATGTCGTCAA1498920.15777020136320308No Hit
CTACAACGAGGCAGTA1480550.15583665014029457No Hit
GCATCAATGTGCATCA1464730.15417150150956985No Hit
CCTAACGCTAATCGCT1464350.15413150426053854No Hit
CGATAAAGATGACTCA1415740.14901501406208545No Hit
ACGTAGGGATCGTAGT1414190.14885186738840508No Hit
TCGCGGTGAGTCGGTC1395310.14686463564705982No Hit
TAGTTGCGACTCACTC1395020.1468341114306938No Hit
CTTCAGCACAAGTTAG1386980.145987853845926No Hit
TTACCATTGTGAGCAG1383900.14566366561693536No Hit
GTTGAGTTGTGCAAGG1379230.1451721204775242No Hit
GGACCTCGAACGTGCC1378620.1451079143672371No Hit
TGAGGCCACATAACCC1347390.14182077203237412No Hit
GGGTGTGCTCGACATT1326500.13962197589483688No Hit
TCGGCTTACTGTCACA1318290.13875782480392349No Hit
TTTACCAACCAGTCCG1296400.13645377274788278No Hit
GGCGTTTCTTTCGCCT1272210.13390763207928416No Hit
AAAGGGAGACGCACTG1262560.1328919124657258No Hit
CCGACAGTGGCGAGTA1253150.13190145427260824No Hit
ACTACGCCTGCTCCTT1241160.1306394358097518No Hit
TAGGATAGAGGACAGT1213710.12775016084683186No Hit
TTGTTAGCTCCTCCTA1205210.12685548553955248No Hit
ACAGTTGACTTCGCAA1193780.12565241039105796No Hit
CTTACATTGGTTGACT1193410.12561346570121168No Hit
AAGACCTACCCAGGCT1192990.1255692582154402No Hit
GCCGTTTACTTGTGAC1183960.12461879727135398No Hit
GTTGTGATGTCTTTGT1180880.12429460904236334No Hit
CAGTGATTGAACGCAG1173970.12356729064550444No Hit
TGTGTAGTGAGGCATG1172280.12338940814323361No Hit
TAGTTGCGACTCGATA1167150.12284944528131085No Hit
CGCTTACTGAACGCAG1166890.12282207874249997No Hit
CGTGTAATGTGGCTTA1159580.12205265797823968No Hit
TGCTAAGACCGGTTGC1150750.12112324821785414No Hit
AGCACATTGTAGGAGC1148100.12084432003381998No Hit
GCTTCTCGAAAAGGTT1138670.11985175672233238No Hit
TTGTACTACCCTGAGC1113780.1172319369107813No Hit
ATGGGTACTCCTAGCG1109340.11676460063262596No Hit
GTTATGCGATCATGCA1104000.1162025340278175No Hit
CTTTCCGGATTCGTTT1100350.11581834992527987No Hit
AAGGCGATGTAGCAGG1076730.11333220513022821No Hit
CCAAGTTGAACCTAGT1075590.11321221338313427No Hit
AGCTGCTCTAGCTTTG1070970.11272593103964829No Hit
AGCGCCTGATGTTGTG1067520.11236279812081136No Hit
CGCATCGGAATCAGCT1062500.111834413409924No Hit
CGCAAAGGAGTTGACT1059880.11155864290344493No Hit
GGTACGCTGGGCAGTA1058850.11145022930738636No Hit
CAAGTGGGAAGTTGAA1056590.11121235093156856No Hit
TCAGTCCACTGGCTTA1055730.1111218308416556No Hit
CAACGAACTTTATGGC1042200.10969771826430379No Hit
TAGTACCGATTTCCGC1035050.10894513844700408No Hit
TAACTCACTTATTGCG1025140.10790205229463481No Hit
GCGGCTATGGGCAGTA1022560.10763049202489589No Hit
GATCTGCCTGTACACT1011680.10648530763157826No Hit
TCTTGAGGACTCACGG1009440.10624953437413052No Hit
GTTGATGACAATCGCT988680.10406442150599872No Hit
CCGATTCACCCAGAGA988520.10404758055903819No Hit
TTCTATGACATCAGGG984540.1036286620033944No Hit
GCTGACCGACTGTGCG979250.10307185819451113No Hit
AGATGTAGACCTAGGC973830.10250137111622239No Hit
CTCTGACTGGTGAGCA964000.10146670543733338No Hit
GTAATCGACGAGCTGA956720.10070044235062822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)