FastQCFastQC Report
Wed 29 May 2024
EGAF00008431395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13984603
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTAT285940.2044677278289559RNA PCR Primer, Index 27 (100% over 41bp)
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC183430.1311656827154836RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC177250.1267465368877472RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)