FastQCFastQC Report
Wed 29 May 2024
EGAF00008431406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10859440
Total Bases814.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGCTGCCACCATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC592280.545405656276935RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC246330.22683490124720979RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC218630.20132714025769285RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC180900.16658317555969737RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC165000.15194153658015513RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA164500.1514811076814274RNA PCR Primer, Index 1 (100% over 32bp)
CCACCGCTGCCACCATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC147410.13574364792291316RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC143940.13254827136574263RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC131590.12117567756716736RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC130010.11972072224718769RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC128850.11865252720213934RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)