FastQCFastQC Report
Wed 29 May 2024
EGAF00008431415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11995877
Total Bases899.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC477980.39845356867196957RNA PCR Primer, Index 1 (100% over 31bp)
CGGGTGCCCCCTCCTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA215240.17942831524531305RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCCCCCTCCTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA208510.17381805431983005RNA PCR Primer, Index 1 (100% over 32bp)
CCACTCCTGACACCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC193400.16122205987940688RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA183040.1525857592571181RNA PCR Primer, Index 1 (100% over 32bp)
CCACTCCTGACACCAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC158930.13248718705601933RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCACGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC143400.11954107232009799RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)