FastQCFastQC Report
Wed 29 May 2024
EGAF00008431445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008431445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7927208
Total Bases594.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCGCTGCCACCATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC286850.3618550188162087RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111420.14055389993551323RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111050.14008715300519425RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC92300.11643443694173282RNA PCR Primer, Index 1 (100% over 31bp)
CCACTCCTGACACCACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC83950.10590109405480468RNA PCR Primer, Index 1 (100% over 31bp)
CCACCGCTGCCACCAATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC81290.10254556206926828RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)