FastQCFastQC Report
Wed 24 Jul 2024
EGAF00008437414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008437414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84864
Total Bases11.5 Mbp
Sequences flagged as poor quality0
Sequence length50-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGAGTT5630.6634144042232277No Hit
GTGCAGAGATACATCACAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT4250.500801282051282No Hit
TCTCTGCACTGATCACCTAGGTCTTGTAGCTATTTTCTAGGATCTGCCTA2940.3464366515837104No Hit
GTGCATAGATATGTCACAATATGCCCTGGAAGCAGAGCCTAGACAAGAGT2650.31226432880844646No Hit
ACCCTGTACTGATCATCCAGGTGATGGAGCTTTTGTCTAGGCTCTGCCTA2610.3075509049773756No Hit
GTGCAGAGATATGTCACAAAGCTCCTTTAGGCCGAACTTAGATGTGTTAC2360.2780920060331825No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGTGTT2160.25452488687782804No Hit
TCTATGCACTGATCACCTTAGTGATGTAACACTTTGGTAGGCTCGGCCTA2070.24391968325791855No Hit
GTGCAGAGATATGTGACAAGGCCCCTTGAAGCAGAGCCTAGACAATACTT1970.23213612368024134No Hit
TCTCTGCACTGATCTCCCAGGTGCTGTAACTTTAGTCTAGGCTCTGGCTA1620.19089366515837103No Hit
TCTCTGCACTGATCACTTAGGTGATGTAACACTTTTATAATCACTGCCTA1610.1897153092006033No Hit
TCTCTGCACTGATCACCCAGGTGATGTAACTCTTGTCTAGGCTCTGCTTC1530.18028846153846154No Hit
TCACTGCACTGATCACCCAGGCGATATAACTCTTCTCTAGGATCTGACCA1520.17911010558069382No Hit
TCTCTGCACTGATCCCGAGGTGATACAACTCTTGCCTGGTCTCTGCCTAC1420.16732654600301658No Hit
ATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTATCTCT1390.16379147812971342No Hit
GTGCAGAGATATGTCAAGATTCCCCTGTAGGCCGAGCCTACCAAAGTGTT1310.15436463046757165No Hit
GGTGATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTAT1310.15436463046757165No Hit
GTGCAGAGATATGTCTCAATCCCCCTGTGGGCACAGCCTAGACAAGAGTT1280.1508295625942685No Hit
TCACTAAGGTGATCAGTGCATAGATATGTCACAATATGCCCTGGAAGCAG1270.14965120663650078No Hit
TCCCTGCAATGATCACCCAGGTGATATACCACTTGCCAAGGCTCTGCCTA1250.1472944947209653No Hit
GTGCAGAGATTTCTCACAATGCCCCCATAGGCAGATCCGAGAGAAGAGTT1240.1461161387631976No Hit
GTGCAGTGATATGTCACAATGCCGTGTAGCCAGAGCCTAGACTAAAGTTA1230.14493778280542988No Hit
TCTCTGCACTGATCACCTAGGTCATGTAACTTTTTTCTAGGCTCTACCTA1190.14022435897435898No Hit
TCTCTGCACTGATCACCTAGGTGATGTAACTCTTGTCTACCCTCTGTCCA1160.1366892911010558No Hit
GTATAGAATATATACCTTGGGTACTTTGATATTTTATGTACAGTATATAA1130.13315422322775264No Hit
TGTCTAGGATGTGCCTAAATTAACTTTTTGACATAACCCTGTACTGATCA1130.13315422322775264No Hit
GTGCAGAGATTTGTCAAAATTCCCTGTAGGCAGTGATTATAAAAGTGTTA1130.13315422322775264No Hit
GCCTAGACAAGAGTTAAATCACCTCGGTTAACAGTGCAGAGATATGTCAA1090.12844079939668174No Hit
TCTCTGCACTGATCACCCAGGTGATTCAACTCTTGACTAGGAACTGCCTA1050.12372737556561085No Hit
GTTCAGGGATATGTGGAAACGCCCCTGTAGGCAGAGCTTGGACAAGGGTT1040.12254901960784313No Hit
GCCTACAGGGGCATTGCAATGTATCTCTGCACTGATCACCTAGGTCTTGT1040.12254901960784313No Hit
TCCTAGACAAGAGTTACATCACTTGGATGATTAGTGCAGAGATATGTCAC1010.11901395173453996No Hit
TCTCTGCACTGATCACCCAGGTGATATAACTCTTCTCTAGGATCTGACCA970.11430052790346909No Hit
GTGCAGAGATATATCGCAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT940.11076546003016591No Hit
GTACATCACCTGGGTAATCCTTGCTGGGACACGTCACAAAGCACCCTGTA930.10958710407239819No Hit
GATCAGTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAA930.10958710407239819No Hit
GTGCAAAGGTATGTCACAAAGCCCCCTGTACGCAAAGCCTAGACAATGGT930.10958710407239819No Hit
GTCTAGGATGTGCCTAAATTAACTTTTTGACATAACCCTGTACTGATCAT850.1001602564102564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)