FastQCFastQC Report
Wed 24 Jul 2024
EGAF00008437437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008437437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75413
Total Bases10.4 Mbp
Sequences flagged as poor quality0
Sequence length50-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTT5440.7213610385477305No Hit
GTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTA4110.5449988728733772No Hit
ATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCT3550.4707411189052285No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGAGTT2920.38720114569106123No Hit
ATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAG2150.2850967339848567No Hit
GTGCATAGATATGTCACAATATGCCCTGGAAGCAGAGCCTAGACAAGAGT2120.2811186400222773No Hit
GTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAGAAGCGCAG1950.25857610756766075No Hit
GTATGAATATACCTTCAAAGCAGCAACACAGGTTCCCCATGAGAAAAAGC1870.24796785700078233No Hit
GTGCAGAGATACATCACAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT1860.24664182567992257No Hit
GTATATTATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTT1790.23735960643390397No Hit
GTGCAGAGATATGTCACAAAGCTCCTTTAGGCCGAACTTAGATGTGTTAC1760.23338151247132458No Hit
GTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAGAAGC1700.2254253245461658No Hit
TCTCTGCACTGATCACCTAGGTCTTGTAGCTATTTTCTAGGATCTGCCTA1640.217469136621007No Hit
GCACAGACAGTGGTCCTTAGGACGAAGAGCAGGAGACCCCTAATTCCGTC1380.18299232227865223No Hit
GAATATACCTTCAAAGCAGCAACACAGGTTCCCCATGAGAAAAAGCAAAC1350.17901422831607283No Hit
ACCCTGTACTGATCATCCAGGTGATGGAGCTTTTGTCTAGGCTCTGCCTA1340.17768819699521302No Hit
ATATGATACTGTATATTATTATATACATAATGTATACGTATGTTTGTGGG1210.16044978982403565No Hit
GGTGATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTAT1180.15647169586145626No Hit
GTGCAGAGATATGTGACAAGGCCCCTTGAAGCAGAGCCTAGACAATACTT1170.15514566454059645No Hit
CCTTAGGACGAAGAGCAGGAGACCCCTAATTCCGTCACCATGGCGATAGG1090.14453741397371805No Hit
TACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAAC1070.14188535133199848No Hit
TTATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCAT1060.14055932001113866No Hit
GGCATAAACATTGCTGGGTGAGGGCACAATCCACATTGTGAGGTCCAACT1020.13525519472769948No Hit
ACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACT1000.13260313208597987No Hit
GTGCAGAGATTTCTCACAATGCCCCCATAGGCAGATCCGAGAGAAGAGTT990.13127710076512009No Hit
CTTCTGACCTTTGAAAGACACAGCACAGACAGTGGTCCTTAGGACGAAGA940.12464694416082107No Hit
TCTCTGCACTGATCTCCCAGGTGCTGTAACTTTAGTCTAGGCTCTGGCTA940.12464694416082107No Hit
ATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAA920.12199488151910147No Hit
TCCCTGCAATGATCACCCAGGTGATATACCACTTGCCAAGGCTCTGCCTA900.11934281887738189No Hit
TCACTAAGGTGATCAGTGCATAGATATGTCACAATATGCCCTGGAAGCAG900.11934281887738189No Hit
GTGCAAAGGTATGTCACAAAGCCCCCTGTACGCAAAGCCTAGACAATGGT870.1153647249148025No Hit
CTTTTACATAGACCGAAAGGTCATTGAAGGCTCAGAGTTTTGTTTCAAGA850.1127126622730829No Hit
TCTCTGCACTGATCACCTAGGTCATGTAACTTTTTTCTAGGCTCTACCTA840.1113866309522231No Hit
GCCTACAGGGGCATTGCAATGTATCTCTGCACTGATCACCTAGGTCTTGT840.1113866309522231No Hit
TCACTGCACTGATCACCCAGGCGATATAACTCTTCTCTAGGATCTGACCA810.1074085369896437No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGTGTT800.1060825056687839No Hit
TCTATGCACTGATCACCTTAGTGATGTAACACTTTGGTAGGCTCGGCCTA790.10475647434792411No Hit
TCTCTGCACTAATCATCCAAGTGATGTAACTCTTGTCTAGGATGTGCCTA770.1021044117062045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)