FastQCFastQC Report
Wed 24 Jul 2024
EGAF00008437440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008437440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75413
Total Bases9.3 Mbp
Sequences flagged as poor quality0
Sequence length50-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTT4670.6192566268415259No Hit
GTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTA3330.44156842984631295No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGAGTT2730.3620065505947251No Hit
ATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCT2600.34476814342354767No Hit
GTATATTATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTT2360.3129433917229125No Hit
GTATGAATATACCTTCAAAGCAGCAACACAGGTTCCCCATGAGAAAAAGC1860.24664182567992257No Hit
ATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAG1760.23338151247132458No Hit
TCTCTGCACTGATCACCTAGGTCTTGTAGCTATTTTCTAGGATCTGCCTA1700.2254253245461658No Hit
GTGCATAGATATGTCACAATATGCCCTGGAAGCAGAGCCTAGACAAGAGT1620.2148170739792874No Hit
GCACAGACAGTGGTCCTTAGGACGAAGAGCAGGAGACCCCTAATTCCGTC1480.1962526354872502No Hit
GTGCAGAGATACATCACAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT1450.1922745415246708No Hit
GAATATACCTTCAAAGCAGCAACACAGGTTCCCCATGAGAAAAAGCAAAC1410.18697041624123162No Hit
GTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAGAAGCGCAG1300.17238407171177383No Hit
GTGCAGAGATATGTCACAAAGCTCCTTTAGGCCGAACTTAGATGTGTTAC1280.16973200907005423No Hit
ACCCTGTACTGATCATCCAGGTGATGGAGCTTTTGTCTAGGCTCTGCCTA1260.16707994642833462No Hit
CTTCTGACCTTTGAAAGACACAGCACAGACAGTGGTCCTTAGGACGAAGA1200.15912375850317584No Hit
TCTCTGCACTGATCTCCCAGGTGCTGTAACTTTAGTCTAGGCTCTGGCTA1140.15116757057801705No Hit
TCACTGCACTGATCACCCAGGCGATATAACTCTTCTCTAGGATCTGACCA1140.15116757057801705No Hit
TCACTAAGGTGATCAGTGCATAGATATGTCACAATATGCCCTGGAAGCAG1140.15116757057801705No Hit
GGCATAAACATTGCTGGGTGAGGGCACAATCCACATTGTGAGGTCCAACT1100.14586344529457787No Hit
TCTATGCACTGATCACCTTAGTGATGTAACACTTTGGTAGGCTCGGCCTA1060.14055932001113866No Hit
GTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAGAAGC1040.13790725736941908No Hit
TTATTATATACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCAT960.1272990068025407No Hit
GTGCAGAGATTTCTCACAATGCCCCCATAGGCAGATCCGAGAGAAGAGTT940.12464694416082107No Hit
GGTGATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTAT930.12332091283996129No Hit
TACATAATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAAC890.11801678755652208No Hit
GTGCAAAGGTATGTCACAAAGCCCCCTGTACGCAAAGCCTAGACAATGGT880.11669075623566229No Hit
GTGCAGAGATATGTGACAAGGCCCCTTGAAGCAGAGCCTAGACAATACTT870.1153647249148025No Hit
TCCCTGCAATGATCACCCAGGTGATATACCACTTGCCAAGGCTCTGCCTA870.1153647249148025No Hit
GGTTCAATCTCTTCTGACCTTTGAAAGACACAGCACAGACAGTGGTCCTT860.11403869359394268No Hit
GCCTACAGGGGCATTGCAATGTATCTCTGCACTGATCACCTAGGTCTTGT850.1127126622730829No Hit
ATATGATACTGTATATTATTATATACATAATGTATACGTATGTTTGTGGG810.1074085369896437No Hit
CCCTTAGGCAGAGCCTAGACAAAAGCTCCATCACCTGGATGATCAGTACA810.1074085369896437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)