FastQCFastQC Report
Wed 24 Jul 2024
EGAF00008437471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008437471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176473
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length50-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGAGTT9610.5445592243572671No Hit
GTGCAGAGATACATCACAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT5680.32186226788233896No Hit
GTGCAGAGATATGTCACAAAGCTCCTTTAGGCCGAACTTAGATGTGTTAC5470.3099624305134497No Hit
GTGCATAGATATGTCACAATATGCCCTGGAAGCAGAGCCTAGACAAGAGT5140.29126268607662364No Hit
ACCCTGTACTGATCATCCAGGTGATGGAGCTTTTGTCTAGGCTCTGCCTA4680.2651963756495328No Hit
TCTCTGCACTGATCACCTAGGTCTTGTAGCTATTTTCTAGGATCTGCCTA4340.24592997229037872No Hit
TCTCTGCACTGATCTCCCAGGTGCTGTAACTTTAGTCTAGGCTCTGGCTA3880.21986366186328787No Hit
GTGCAGAGATATGTGACAAGGCCCCTTGAAGCAGAGCCTAGACAATACTT3450.19549732820318125No Hit
GTGCAGTGATATGTCACAATGCCGTGTAGCCAGAGCCTAGACTAAAGTTA3310.18756410329058837No Hit
TCTATGCACTGATCACCTTAGTGATGTAACACTTTGGTAGGCTCGGCCTA3090.175097606999371No Hit
TCTCTGCACTGATCCCGAGGTGATACAACTCTTGCCTGGTCTCTGCCTAC3050.17283097131005876No Hit
GCCTAGACAAGAGTTAAATCACCTCGGTTAACAGTGCAGAGATATGTCAA2680.15186459118392048No Hit
GTGCAGAGATTTCTCACAATGCCCCCATAGGCAGATCCGAGAGAAGAGTT2610.14789797872762406No Hit
GTGCAGAGATTTGTCAAAATTCCCTGTAGGCAGTGATTATAAAAGTGTTA2600.14733131980529598No Hit
TCACTGCACTGATCACCCAGGCGATATAACTCTTCTCTAGGATCTGACCA2430.13769811812571897No Hit
TCACTGCACTGATCAACCAGGTGATATAACTCTTGTATAGGCTCTGCCTA2290.1297648932131261No Hit
GTGCAGAGATATTTCACAATGCCCCGTTGGACAGAGGGTAGACAAGAGTT2270.12863157536846997No Hit
TCACTAAGGTGATCAGTGCATAGATATGTCACAATATGCCCTGGAAGCAG2200.12466496291217352No Hit
TCCCTGCAATGATCACCCAGGTGATATACCACTTGCCAAGGCTCTGCCTA2110.11956503261122099No Hit
GTGCAGAGATATGTCTCAATCCCCCTGTGGGCACAGCCTAGACAAGAGTT2030.1150317612325965No Hit
TGTCTAGGCTGTGCCCACAGGGGGATTGAGACATATCTCTGCACTGATCC2020.11446510231026842No Hit
TGTCTAGGATGTGCCTAAATTAACTTTTTGACATAACCCTGTACTGATCA1990.11276512554328423No Hit
GGTGATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTAT1880.10653187739767557No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGTGTT1800.10199860601905106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)