FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008459028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008459028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25649695
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAACCTCTAACGATCGCGTAATAGATCGGAAGAGCACACGTCTGAACT629090.2452621756321079Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCAACCTCTAACGATCGCGTAATAGATCGGAAGAGCAGACGTCTGAACT482100.18795545132213073Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGCAACCTCTAACGATCGCGTAATAGATCGGAAGAGCAGTCGTCTGAACT272090.10607923408056119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)