FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008459128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008459128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355287
Total Bases203.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG35460.26164199907473473Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG24260.17900267618592963Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)