FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008459353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008459353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5984435
Total Bases897.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACAGGGTTTGGCTAAGCATTGCAAGATCTGAAGAGCACACGTCTGAA169880.2838697387472669Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGATGTGAACACGTC143460.2397218785064923No Hit
CGAACAGGGTTTGGCTAAGCATTGCAAGATCTGAAGAGCACAAGTCTGAA129400.21622759709145475No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC102150.17069280558649227No Hit
GTCATCGGGATTTACGAAATTCGAAGATCGGAAGAGCACACGTCTGAACT97390.16273883833645114Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAGGGTAGGTTTATCTTCGCACGAGATCGGAAGAGCACACGTCTGAACT94320.15760886366047924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAACAGGGTTTGGCTAAGCATTGCAAGATCGGAAGAGCACACGTCTGAA86880.14517661232848214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAACAGGGTTTGGCTAAGCATTGCAAGATCAGAAGAGCACAAGTCTGAA81810.13670463460627444No Hit
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGAAGTGAACACGTC73340.12255125170546594No Hit
CGAACAGGGTTTGGCTAAGCATTGCAAGATCAGAAGAGCACACGTCTGAA69580.11626828597854266Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC61320.10246581339758891No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCAGAAGAGCACAAGTCTGAA60110.10044390155461627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)