FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008459479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008459479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053313
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCGTCGTCTCGGGAA375990.5330686444795517No Hit
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCGTCGTCTAGGGAA361880.5130638609118864Illumina Single End PCR Primer 1 (96% over 26bp)
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCGTCGTGTAGGGAA238410.33801137139384Illumina Single End PCR Primer 1 (100% over 26bp)
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCTTCGTCTAGGGAA219950.31183927326066485No Hit
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCTTCGTCTCGGGAA174640.2475999576369289No Hit
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCGTCGTCTAGGCAA124540.17656950712381544Illumina Single End PCR Primer 1 (95% over 23bp)
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCTTCGTCTAGGCAA100380.14231610024962738No Hit
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCGTCGTGTCGGGAA85610.12137558619616058Illumina Single End PCR Primer 1 (96% over 26bp)
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAATAGCGTCGTCTCGGGAA80540.1141874747370491No Hit
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAAGAGCTTCGTGTAGGGAA80140.11362036535171485Illumina Single End PCR Primer 1 (96% over 26bp)
ATAGGCCCCCTGCGTGTTCTGAGAAGATCGGAATAGCGTCGTCTAGGGAA75630.10722620703207131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)