FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008459955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008459955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6250455
Total Bases937.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG265680.42505705584633435Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG119640.19141006534724272No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG102560.16408405468081924Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA101910.1630441303873078Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG75240.12037523668276949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)