FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008460043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008460043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16213663
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGAATCGAATAAAGACGGCCGATCCAGATCGGAAGTGCACACGTCTG3094051.9082979583330428Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTTGAATCGAATAAAGACGGCCGATCCAGATCGGAAGTGCAAACGTCTG1456710.8984459588188061No Hit
CCTTGAATCGAATAAAGACGGCCGATCCAGAACGGAAGTGCACACGTCTG1417090.8740097780495375No Hit
CCTTGAATCGAATAAAGACGGCCGATCCAGAACGGAAGTGCAAACGTCTG828500.5109887876663034No Hit
CCTTGAATCGAATAAAGACGGCCGATCCCGATCGGAAGTGCACACGTCTG521780.3218150025691295Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CCTTGAATCGAATAAAGACGGCCGATCCCGAACGGAAGTGCACACGTCTG413910.25528469414961935No Hit
CCTTGAATCGAATAAAGACGGCCGATCCCGATCGGAAGTGCAAACGTCTG303710.18731732613413762No Hit
CCTTGAATCGAATAAAGACGGCCGATCCCGAACGGAAGTGCAAACGTCTG281570.17366217615353174No Hit
CCTTGAATCGAATAAAGACGGCCGATCCAGATCGGAAGTGGACACGTCTG194110.11972001638371292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)