FastQCFastQC Report
Wed 24 Jul 2024
EGAF00008477585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008477585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150143
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length50-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGAGTT6860.4568977574712108No Hit
GTGCAGAGATACATCACAATGTGCCTGTAGGCAGAGCCTTGATGAGAGGT5210.3470025242602053No Hit
TCTCTGCACTGATCACCTAGGTCTTGTAGCTATTTTCTAGGATCTGCCTA4260.28372951119932327No Hit
GTGCATAGATATGTCACAATATGCCCTGGAAGCAGAGCCTAGACAAGAGT4240.28239744776646264No Hit
GTGCAGAGATATGTCACAAAGCTCCTTTAGGCCGAACTTAGATGTGTTAC4170.27773522575145027No Hit
TCTCTGCACTGATCTCCCAGGTGCTGTAACTTTAGTCTAGGCTCTGGCTA3100.2064698320934043No Hit
ACCCTGTACTGATCATCCAGGTGATGGAGCTTTTGTCTAGGCTCTGCCTA3020.20114157836196161No Hit
GTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAAGTGTT2900.19314919776479755No Hit
GTGCAGAGATATGTGACAAGGCCCCTTGAAGCAGAGCCTAGACAATACTT2580.1718361828390268No Hit
TCCCTGCAATGATCACCCAGGTGATATACCACTTGCCAAGGCTCTGCCTA2570.17117015112259645No Hit
TCTATGCACTGATCACCTTAGTGATGTAACACTTTGGTAGGCTCGGCCTA2560.17050411940616614No Hit
TCTCTGCACTGATCACCCAGGTGATGTAACTCTTGTCTAGGCTCTGCTTC2520.1678399925404448No Hit
TCACTAAGGTGATCAGTGCATAGATATGTCACAATATGCCCTGGAAGCAG2500.1665079291075841No Hit
TCTCTGCACTGATCCCGAGGTGATACAACTCTTGCCTGGTCTCTGCCTAC2440.16251173880900208No Hit
GGTGATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTAT2420.16117967537614142No Hit
GTGCAGAGATTTCTCACAATGCCCCCATAGGCAGATCCGAGAGAAGAGTT2370.15784951679398973No Hit
ATGTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTT2340.15585142164469873No Hit
TCTCTGCACTGATCACCCAGGTGATTCAACTCTTGACTAGGAACTGCCTA2300.15318729477897738No Hit
GTGCAAAGGTATGTCACAAAGCCCCCTGTACGCAAAGCCTAGACAATGGT2090.1392006287339403No Hit
GTGCAGAGATATGTCAAGATTCCCCTGTAGGCCGAGCCTACCAAAGTGTT1860.12388189925604258No Hit
ATATACCACTTGCCAAGGCTCTGCCTACAGGGGCATTGCAATGTATCTCT1850.12321586753961224No Hit
TCACTGCACTGATCACCCAGGCGATATAACTCTTCTCTAGGATCTGACCA1810.12055174067389089No Hit
GTGCAGAGATTTGTCAAAATTCCCTGTAGGCAGTGATTATAAAAGTGTTA1790.11921967724103022No Hit
GATCAGTACAGGGTTATGTCAAAAAGTTAATTTAGGCACATCCTAGACAA1790.11921967724103022No Hit
GCCTAGACAAGAGTTAAATCACCTCGGTTAACAGTGCAGAGATATGTCAA1770.11788761380816955No Hit
GTGCAGAGATATGTCTCAATCCCCCTGTGGGCACAGCCTAGACAAGAGTT1740.11588951865887853No Hit
ACCTACGATGGCATTGTGACATATCTCTGCACTAATCATCCAAGTGATGT1730.1152234869424482No Hit
GCCTACAGGGGCATTGCAATGTATCTCTGCACTGATCACCTAGGTCTTGT1730.1152234869424482No Hit
TGTCTAGGATGTGCCTAAATTAACTTTTTGACATAACCCTGTACTGATCA1710.11389142350958753No Hit
TCTCTGCACTGATCACTTAGGTGATGTAACACTTTTATAATCACTGCCTA1700.11322539179315719No Hit
CCTCTGCACTGATCACCCAGGTGATATAACTCTTCTCTAGGATCTGCCTA1670.11122729664386619No Hit
TCCTAGACAAGAGTTACATCACTTGGATGATTAGTGCAGAGATATGTCAC1660.11056126492743584No Hit
TCTCTGCACTAATCATCCAAGTGATGTAACTCTTGTCTAGGATGTGCCTA1650.1098952332110055No Hit
TCTCTGCACTGATCACCTAGGTCATGTAACTTTTTTCTAGGCTCTACCTA1640.10922920149457518No Hit
GTACATCACCTGGGTAATCCTTGCTGGGACACGTCACAAAGCACCCTGTA1620.1078971380617145No Hit
GTATACGTATGTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTA1600.10656507462885384No Hit
TCTCTGCACTGATCACCTCGGTCATGTAACTTTTTTCTAGGCTCTACCTA1510.10057078918098081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)