FastQCFastQC Report
Thu 25 Jul 2024
EGAF00008487531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131879394
Total Bases19.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT4368420.33124356030935354TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT1331410.1009566361822985TruSeq Adapter, Index 27 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1326050.10055020422674978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)