FastQCFastQC Report
Thu 25 Jul 2024
EGAF00008487548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87296462
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length80
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG2201130.25214423924763407No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1155880.13240857344252965No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1024090.11731174168318528TruSeq Adapter, Index 9 (100% over 50bp)
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG929780.10650832561805311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)