FastQCFastQC Report
Wed 31 Jul 2024
EGAF00008487593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115682693
Total Bases17.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT5657100.4890186987607559TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT2091200.18077034219803303TruSeq Adapter, Index 2 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1369990.11842653075166568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)