FastQCFastQC Report
Wed 31 Jul 2024
EGAF00008487621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106807276
Total Bases16.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2494380.2335402692977583TruSeq Adapter, Index 7 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1849060.17312116451691925No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTAT1742710.1631639777050395TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)