FastQCFastQC Report
Wed 31 Jul 2024
EGAF00008487652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108550776
Total Bases16.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2233830.20578664495222035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1408090.12971717493756102TruSeq Adapter, Index 27 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1282330.11813181326313135No Hit

[FAIL]Adapter Content

Adapter graph