FastQCFastQC Report
Wed 31 Jul 2024
EGAF00008487665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73975222
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2789730.3771168135189915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1828520.24718006253499314TruSeq Adapter, Index 7 (97% over 35bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG1038660.1404064728592501No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG881850.1192088345473299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)