FastQCFastQC Report
Wed 31 Jul 2024
EGAF00008487693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008487693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244883505
Total Bases36.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT15990390.6529794646642288TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11017600.449911887695335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT5789380.23641363676169205TruSeq Adapter, Index 7 (97% over 35bp)
GCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG3532600.1442563475232846No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG3326150.13582580827565335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)