FastQCFastQC Report
Tue 20 Aug 2024
EGAF00008502720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008502720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30607871
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC1342100.43848198393151877No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT1207200.39440835332846247No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC1207000.394343010658925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1202670.39292834186343767No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG941390.3075646783796233No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG929750.3037617350125398No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA906100.2960349643397282No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG898080.29341472329127366No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA884110.288850537824078No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA880660.2877233767745558No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC860970.2812903909585871No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC836020.27313889293378163No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA788220.2575219949143147No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA773280.252640897499862No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCC769210.25131117417477356No Hit
GTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAA721090.23558972788404656No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC711820.23256109515098258No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA703230.22975462749434614No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT667310.21801908404540782No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC654380.2137946804598072No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC653370.21346469997864273No Hit
GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTC644640.21061249245333005No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA640280.20918802225741215No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA623040.20355548414327804No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG615750.2011737438386355No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT609250.19905010707866613No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG606120.19802749430040395No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT602830.19695260738651182No Hit
GGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGG595400.19452512721319296No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG584850.19107830139508888No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA569630.1861057242432837No Hit
GCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACC567340.1853575506770791No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGAC555420.181463127572643No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA554830.18127036669750732No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACA533980.174458393398221No Hit
ACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCG531220.17355666455860325No Hit
GGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCAT506530.16549011200419655No Hit
GAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAG504270.16475173983842262No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG495890.16201388198480057No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTA489900.16005686903215188No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC489650.15997519069523No Hit
GCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAAC479120.15653489914407964No Hit
GCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTC474950.15517250448422237No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATT471340.15399306929907017No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC467780.15282996978130234No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC464380.1517191443991645No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC462190.15100364216772869No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC454430.14846834658967298No Hit
GTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCAC446450.14586117407512597No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA444540.14523715158104267No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC441730.14431908707404054No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT440460.14390416112247728No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCC438320.14320499455842584No Hit
CATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGAT438270.14318865889104146No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA429830.14043119823655817No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA421210.13761492917949114No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT418000.13656617933341395No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC417440.1363832198587089No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG411400.13440987123867582No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA411350.13439353557129144No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC407030.13298213390928104No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACAT402150.1313877727725656No Hit
GGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCG401340.13112313496093866No Hit
GACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGG400300.13078335307934355No Hit
GAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACA397750.1299502340427402No Hit
GTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCG396870.12966272629677508No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA395900.12934581434951814No Hit
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC395540.1292281975443506No Hit
GGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCA392240.12815004349698156No Hit
GGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTT388290.12685952577361556No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT388210.12683338870580055No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC383130.12517368489954758No Hit
ACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTC382680.12502666389308817No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG382260.12488944428705936No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT375930.1228213487961969No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG374680.1224129571115874No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC372070.12156023527412278No Hit
GGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA370750.12112897365517517No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC366690.11980251746356353No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTA359320.11739464009110596No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC357170.11669220639357765No Hit
GAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAAT353930.11563365514706986No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT349440.11416671221595256No Hit
GTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGC349390.11415037654856819No Hit
GTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTCTCGTGCC343610.1122619733989339No Hit
GGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCA342930.11203980832250632No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGA342660.11195159571863067No Hit
CCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACG342040.11174903344306436No Hit
GTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGC341460.11155953970140556No Hit
CCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAAC338130.11047158425360587No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC330060.10783500753776701No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT328720.10739721165186564No Hit
GCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGAT328010.10716524517500745No Hit
GCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCT326980.10682873042688922No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGG324670.10607402259373087No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG324010.10585839178425707No Hit
GCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCT322670.1054205958983557No Hit
GTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTC321110.10491092307596304No Hit
CGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGC318840.10416928377671221No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA317300.1036661452212733No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG316970.1035583298165364No Hit
GCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTA315130.10295717725679124No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC314190.1026500667099649No Hit
GAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACC309260.10103936990586507No Hit
CCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTT309190.10101649997152694No Hit
CCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGA308170.10068325235688558No Hit
AGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTC306960.1002879292061836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)