FastQCFastQC Report
Tue 20 Aug 2024
EGAF00008503670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008503670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20606185
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC2652621.287293111267321No Hit
CCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGG1844720.8952263604349858No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC1270010.6163246617459758No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG848560.41179869053878726No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC710600.3448479182342583No Hit
GCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGG695530.3375345800302191No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT656240.3184674892514068No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC651440.31613809154872674No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT632400.30689814732809595No Hit
CTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCG632320.3068593240330513No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG619120.3004534803506811No Hit
CCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCC571610.27739729600602925No Hit
CCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCG571460.2773245023278205No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA566490.2749126051231705No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG544700.2643381101353793No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCG529770.2570927126976682No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC489830.23771018264661797No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG484150.2349537286984466No Hit
CGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCA479610.23275050670466174No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTC477830.23188668838991788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG469280.22773744873201907No Hit
GCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAA469190.2276937725250938No Hit
CGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCG469150.22767436087757145No Hit
CCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCG440590.21381444454662518No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG437450.212290630216122No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG435360.21127637163308008No Hit
GGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTT433220.21023784849063523No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA426770.2071077203276589No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT412650.2002554087522751No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT383650.18618196429858316No Hit
GGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGC381210.1849978537997208No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT381040.18491535429775088No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG379720.1842747699295139No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG376900.18290624877918935No Hit
CCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAA374690.18183375525358042No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC369240.17918891827866246No Hit
CACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAA359460.17444277045945186No Hit
CGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGG357700.17358865796846917No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTT347350.16856589417206533No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGT341230.16559591210114827No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC334010.16209210972336704No Hit
CCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAG333460.16182519956993496No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG331720.16098079290271344No Hit
GCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTC324200.1573314031685147No Hit
CTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACA320830.1556959718647581No Hit
CCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG316710.1536965721699577No Hit
CGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT315860.15328407466010813No Hit
GACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACG305530.14827101668746545No Hit
GGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCA292000.14170502691303605No Hit
CCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGA289720.14059856300426304No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA284710.13816725415209075No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC277510.1346731575980707No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCC274440.13318331365073155No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGC270680.1313586187836322No Hit
AGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTAC269580.130824798476768No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT263530.12788878678901505No Hit
CCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGA262950.1276073178999412No Hit
GGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGT259000.12569041770711076No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC257140.12478777609732222No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGG256500.12447718973696491No Hit
CGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGA255230.12386086992813079No Hit
CCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGAC247130.11993001130485824No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA243950.1183867853268327No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC243100.1179742878169831No Hit
CAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAAC237120.11507224651239423No Hit
TCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCC235480.11427636896397854No Hit
CTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAG231250.11222358723849174No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG230950.11207799988207424No Hit
CTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCG230940.11207314697019366No Hit
CCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACC227350.11033095160506422No Hit
GCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATC223960.10868581447754642No Hit
CCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAG223130.10828302279145799No Hit
CCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGG217840.10571583240662936No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA214470.10408040110287275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211480.10262938045057832No Hit
CACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGG208790.10132394715470136No Hit
GGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCG206340.10013498374395843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)