FastQCFastQC Report
Thu 19 Sep 2024
EGAF00008552014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008552014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114244
Total Bases4.3 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1206710.562480305311439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT86777.59514722873849No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46684.085991386856202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT39063.418997934246No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA30522.6714750884072687No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23942.0955148629249676No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22852.000105038338994No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20951.8337943349322503No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16611.4539056755715838No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA13811.2088162179195405No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAA13691.1983123840201675No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA13101.1466685340149154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12391.0845208501102903No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAA11360.994362942474003No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA10440.9138335492454747No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9110.7974160568607542No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA8420.7370190119393579No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA6660.5829627814152165No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6260.5479500017506389No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6050.5295682924267358No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.5243163754770491No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAA5830.5103112636112181No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAA5390.47179720598018277No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA5340.46742060852211054No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5080.44466230174013516No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4900.42890655089107527No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT4710.4122754805504009No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAA4370.38251461783550994No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA4320.37813802037743777No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA4280.37463674241098005No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3910.34224992122124576No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAA3630.31774097545604146No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3370.29498266867406603No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3270.2862294737579216No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAA3260.28535415426630717No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAA3180.2783515983333917No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3030.2652218059591751No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.2599698890094885No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGA2940.25734393053464516No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAA2820.24684009663527187No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2820.24684009663527187No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2740.23983754070235636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2740.23983754070235636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2730.23896222121074195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA2680.23458562375266975No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAA2680.23458562375266975No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2670.23371030426105527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2550.223206470361682No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2540.22233115087006758No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.21970519239522426No Hit
CATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA2500.2188298729036098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2420.2118273169706943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2410.21095199747907986No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2410.21095199747907986No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2340.20482476103777877No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2290.20044816357970657No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGA2240.1960715661216344No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAA2170.1899443296803333No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2130.18644305171387557No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAA2130.18644305171387557No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2030.17768985679773117No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA1950.17068730086481568No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1840.16105878645705682No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1800.15755750849059907No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1790.15668218899898462No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGC1750.15318091103252687No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAA1750.15318091103252687No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC1750.15318091103252687No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAGAAAA1690.14792899408284024No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAA1680.14705367459122579No Hit
GTACATGGGGGTATCAACGCAGAAAAAAAAAAAAAAAA1630.14267707713315358No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.13830047967508138No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA1570.13742516018346698No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1570.13742516018346698No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA1500.1312979237421659No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1480.129547284758937No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.11991877035117818No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAGAAAAA1280.11204089492664823No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTA1260.11029025594341935No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA1260.11029025594341935No Hit
GAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAA1230.10766429746857603No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAA1220.10678897797696159No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA1220.10678897797696159No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1210.10591365848534715No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAA1170.10241238051888939No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCA1160.10153706102727494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)