FastQCFastQC Report
Thu 19 Sep 2024
EGAF00008554385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008554385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560239
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT127332.2727800099600346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT80921.444383557731611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT40580.7243337218579927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.6941680247180221No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16390.29255371368291033No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.26863535027015256No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC12770.22793843341859457No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12340.2202631376965902No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11850.2115168704784922No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG10840.19348885029424942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.18849098331247913No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10140.18099418283982371No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC10100.18028020184242796No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC10060.1795662208450322No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG10060.1795662208450322No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC9620.1717124298736789No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG9470.16903500113344483No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC9350.16689305814125757No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9280.165643591395815No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9140.16314465790492988No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA8880.15850378142185745No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG8560.15279193344269143No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG8300.14815105695961903No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8140.145295132970036No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8060.14386717097524448No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGC8040.14351018047654662No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC7890.14083275173631254No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7760.13851231349477633No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7420.13244347501691242No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC7420.13244347501691242No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG7210.12869507478058473No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT7210.12869507478058473No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATG7210.12869507478058473No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6760.12066278855988248No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCC6640.11852084556769522No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6510.11620040732615901No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC6430.11477244533136752No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6400.11423695958332068No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCC6320.11280899758852918No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6190.11048855934699298No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.10816812110545679No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG6060.10816812110545679No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA5960.1063831686119674No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5960.1063831686119674No Hit
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGA5870.10477671136782694No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5810.10370573987173332No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG5780.1031702541236865No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC5710.10192078737824392No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGC5670.10120680638084818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)